TIMP1 antibody (Biotin)
-
- Target See all TIMP1 Antibodies
- TIMP1 (TIMP Metallopeptidase Inhibitor 1 (TIMP1))
-
Reactivity
- Human
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This TIMP1 antibody is conjugated to Biotin
-
Application
- Western Blotting (WB), ELISA
- Specificity
- Human TIMP-1
- Purification
- Immunoaffinity purified
- Immunogen
-
E.coli derived recombinant Human TIMP-1.
Type of Immunogen: Cell extract - Top Product
- Discover our top product TIMP1 Primary Antibody
-
-
- Application Notes
-
Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: ELISA: To detect hTIMP-1 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hTIMP-1. To detect hTIMP-1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human TIMP-1 (LS-B7485) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTIMP-1. Western Blot: To detect hTIMP-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTIMP-1 is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - Comment
-
Target Species of Antibody: Human
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Sterile PBS containing 0.1 % BSA
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS, pH 7.2
- Handling Advice
- Avoid freeze/thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
-
Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
-
- Target
- TIMP1 (TIMP Metallopeptidase Inhibitor 1 (TIMP1))
- Alternative Name
- TIMP1 (TIMP1 Products)
- Synonyms
- TIMP-1 antibody, TIMP1 antibody, DKFZp468A0912 antibody, CLGI antibody, EPA antibody, EPO antibody, HCI antibody, TIMP antibody, Timp antibody, Clgi antibody, TIMP metallopeptidase inhibitor 1 antibody, tissue inhibitor of metalloproteinase 1 antibody, TIMP1 antibody, Timp1 antibody
- Background
-
Name/Gene ID: TIMP1
Synonyms: TIMP1, CLGI, EPA, HCI, Metalloproteinase inhibitor 1, TIMP-1, Collagenase inhibitor - Gene ID
- 7076
- UniProt
- P01033
-