IL-1 beta antibody (Biotin)

Catalog No. ABIN465876

The Rabbit Polyclonal anti-IL-1 beta antibody has been validated for WB, ELISA and Neut. It is suitable to detect IL-1 beta in samples from Mouse.

Quick Overview for IL-1 beta antibody (Biotin) (ABIN465876)

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-1 beta antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-IL-1 beta Antibody (Biotin)

Specificity: Mouse IL-1beta

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Murine IL-1beta.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect mIL-1beta by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-1beta. To detect mIL-1beta by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine IL-1beta (LS-C104813) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-1beta. Western Blot: To detect mIL-1beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1beta is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for IL-1 beta

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Alternative Name: IL1B

Background: Name/Gene ID: IL1B
Family: IL-1

Synonyms: IL1B, IL-1B, IL-1 beta, IL1-BETA, Preinterleukin 1 beta, Pro-interleukin-1-beta, Catabolin, IL-1, IL1F2, Interleukin 1, beta, Interleukin-1 beta

Gene ID: 3553

UniProt: P01584

Pathways: NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome