TNF alpha antibody (APC)

Catalog No. ABIN476326

The APC-conjugated Mouse Monoclonal anti-TNF alpha antibody (Clone MAb11) (ABIN476326) specifically detects TNF alpha in FACS. The antibody is reactive with Human samples.

Quick Overview for TNF alpha antibody (APC) (ABIN476326)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNF alpha antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Clone: MAb11

Product Details anti-TNF alpha Antibody (APC)

Purification: Affinity purified

Immunogen: Human TNF

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: Flo

Usage: This MAb11 antibody is offered in 2 formats:- μg size: has been tested by intracellular staining and flow cytometric analysis of stimulated human peripheral blood cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (ug)/test of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated human peripheral blood cells. This can be used at test size: 5 μL (0.125 μg)/per test. A test is defined as the amount (ug)/test of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Aqueous buffer, 0.09 % sodium azide, may contain carrier protein/stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze. Product is photosensitive and should be protected from light.

Storage: 4 °C

Storage Comment: Store at 4°C. Do not freeze. Protect from light.

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF Alpha

Background: Name/Gene ID: TNF
Family: TNF

Synonyms: TNF, APC1 protein, Cachectin, DIF, TNF Alpha, Tumor necrosis factor-alpha, TNF-alpha, TNF-a, TNFA, Tumor necrosis factor, TNF, macrophage-derived, TNF, monocyte-derived, TNFSF2

Gene ID: 7124

UniProt: P01375

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome