IL-1 beta antibody

Catalog No. ABIN476498

This Mouse Monoclonal antibody specifically detects IL-1 beta in ELISA, FACS, Neut. It exhibits reactivity toward Human.

Quick Overview for IL-1 beta antibody (ABIN476498)

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-1 beta antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Neutralization (Neut)

Clone: CRM56

Product Details anti-IL-1 beta Antibody

Purification: Affinity purified

Immunogen: Human IL1B

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, Flo, Neut

Usage: The CRM56 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human interleukin-1beta (IL-1beta) in combination with the biotin CRM57 (LS-C106945) antibody for detection and recombinant human IL-1beta as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1 - 4 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate. The Functional Grade Purified CRM56 antibody has been tested for neutralization of IL-1beta bioactivity. The fluorochrome-conjugated CRM56 antibody has been tested by intracellular staining for flow cytometric analysis. The fluorochrome- conjugated CRM56 antibody is offered in 2 formats: - μg size: has been tested by intracellular staining with flow cytometric analysis and can be used at less than or equal to 0.50 μg/million cells/100 μL staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested by intracellular staining and flow cytometric analysis of activated human PBMC and can be used at 20 μL per million cells 100 μL blood.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 150 mM sodium chloride, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freezing and store undiluted.

Storage: 4 °C

Storage Comment: Store at 4°C, avoid freeze-thaw cycles.

Target Details for IL-1 beta

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Alternative Name: IL1B

Background: Name/Gene ID: IL1B
Family: IL-1

Synonyms: IL1B, IL-1B, IL-1 beta, IL1-BETA, Preinterleukin 1 beta, Pro-interleukin-1-beta, Catabolin, IL-1, IL1F2, Interleukin 1, beta, Interleukin-1 beta

Gene ID: 3553

UniProt: P01584

Pathways: NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome