RNF7 antibody (Internal Region)

Catalog No. ABIN487985

The Goat Polyclonal anti-RNF7 antibody is suitable to detect RNF7 in samples from Human and Mouse. It has been validated for WB and EIA.

Quick Overview for RNF7 antibody (Internal Region) (ABIN487985)

Target: RNF7 (Ring Finger Protein 7 (RNF7))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This RNF7 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-RNF7 Antibody

Binding Specificity: Internal Region

Specificity: This antibody reacts to RNF7.

Cross-Reactivity (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Peptide with sequence DACLRCQAENKQE, from the internal region of the protein sequence according to NP_055060.1.

Application Details

Application Notes: Peptide ELISA: 1/8000. Western blot: Approx 11 kDa band observed in Human Human Heart lysates (calculated MWof 12.1 kDa according to NP_055060.1). Recommended concentration: 1 - 3 μg/mL. An additional band of 21 kDa was consistently observed, however this band was notblocked by the immunizing peptide and it is therefore a non-specific signal. We call forcaution when used for other assays than Western blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for RNF7

Target: RNF7 (Ring Finger Protein 7 (RNF7))

Alternative Name: RNF7

Background: Synonyms: CKBBP1, CKII beta-binding protein 1, RBX2, RING finger protein 7, RING-box protein 2, ROC2, Regulator of cullins 2, SAG, Sensitive to apoptosis gene protein

Gene ID: 9616

NCBI Accession: NP_055060

UniProt: Q9UBF6

Pathways: Positive Regulation of Endopeptidase Activity