EPH Receptor A2 antibody

Catalog No. ABIN488132

The Mouse Monoclonal anti-EPH Receptor A2 antibody is suitable to detect EPH Receptor A2 in samples from Human. It has been validated for FACS, IF and EIA.

Quick Overview for EPH Receptor A2 antibody (ABIN488132)

Target: EPH Receptor A2 (EPHA2)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EPH Receptor A2 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Clone: GM5H5

Product Details anti-EPH Receptor A2 Antibody

Specificity: Recognizes EphA2 receptor tyrosine kinase (EphA2).

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein G.

Immunogen: Genetic immunisation with cDNA encoding Human EphA2 Remarks: Selection: Based on recognition of the complete native protein expressed on transfected mammalian cells

Isotype: IgG1

Application Details

Application Notes: cELISA: 1/200-1/400. Flow Cytometry: 1.2 μg/10^6 cells. Immunofluorescence: 1 μg/10^6 cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 2.0 mg/mL

Buffer: Phosphate buffered saline, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for EPH Receptor A2

Target: EPH Receptor A2 (EPHA2)

Alternative Name: EPHA2 / ECK

Background: EphA2 (Eph receptor tyrosine kinase A2) belongs to the Eph tyrosine receptor familiy, the largest receptor tyrosine kinase family of transmembrane proteins. It encodes a 130 kDA transmembrane protein which is primarily found in adult human epithelial cells (1). Eph receptors and their ephrin ligands are important mediators of cell-cell communication and play roles in embryonic patterning, neuronal targeting, and vascular development during normal embryogenesis (2,3). The Eph family of receptor tyrosine kinases is frequently overexpressed in a wide variety of cancers and tumor cell lines. In particular, EphA2 is overexpressed in prostate, lung and colon cancers and 40 % of breast cancers and it represent an attractive potential target for drug design (3,4).Synonyms: Eph receptor A2, Ephrin type-A receptor 2, Epithelial cell kinase, Tyrosine-protein kinase receptor ECK

Gene ID: 1969

NCBI Accession: NP_004422

UniProt: P29317

Pathways: RTK Signaling