RECQL2 antibody (AA 122-240)

Catalog No. ABIN4886768

Product Details anti-RECQL2 Antibody

Target: RECQL2 (WRN) (Werner syndrome RecQ like helicase (WRN))

Binding Specificity: AA 122-240

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RECQL2 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Werner syndrome ATP-dependent helicase(WRN) detection. Tested with WB in Human,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Werner syndrome ATP-dependent helicase(WRN) detection. Tested with WB in Human,Rat.
Gene Name: Werner syndrome RecQ like helicase
Protein Name: Werner syndrome ATP-dependent helicase

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human WRN recombinant protein (Position: Q122-N240). Human WRN shares 84% amino acid (aa) sequence identity with mouse WRN.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for RECQL2

Target: RECQL2 (WRN) (Werner syndrome RecQ like helicase (WRN))

Alternative Name: WRN (WRN Products)

Background: Werner syndrome ATP-dependent helicase, also known as DNA helicase, RecQ-like type 3, is an enzyme that in humans is encoded by the WRN gene. This gene encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNA helicases are involved in many aspects of DNA metabolism, including transcription, replication, recombination, and repair. This protein contains a nuclear localization signal in the C-terminus and shows a predominant nucleolar localization. It possesses an intrinsic 3' to 5' DNA helicase activity, and is also a 3' to 5' exonuclease. Based on interactions between this protein and Ku70/80 heterodimer in DNA end processing, this protein may be involved in the repair of double strand DNA breaks. Defects in this gene are the cause of Werner syndrome, an autosomal recessive disorder characterized by premature aging.

Synonyms: Exonuclease WRN | RecQ3 | RECQL2 | RECQL3 | WRN | Q14191

Gene ID: 7486

UniProt: Q14191

Pathways: DNA Damage Repair