MBD3 antibody (C-Term)

Catalog No. ABIN4889329

This Rabbit Polyclonal antibody specifically detects MBD3 in WB, ELISA, IF and ICC. It exhibits reactivity toward Human, Mouse, Rat, Monkey and Hamster.

Quick Overview for MBD3 antibody (C-Term) (ABIN4889329)

Target: MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))

Reactivity: Human, Mouse, Rat, Monkey, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MBD3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-MBD3 Antibody

Binding Specificity: C-Term

Specificity: Reacts with MBD3 from human, mouse, rat, hamster, monkeys and other species (due to sequence homology)

Purification: Purified (protein A)

Immunogen: Synthetic peptide of derived from C terminal part of human MBD3.

Application Details

Application Notes:

Working dilution: Optimal dilutions should be determined by the end user.
The following are guidelines only:
- WB : 1/500 to 1/2000 - ICC, IF: 1/200 to 1/1000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Must be reconstituted in distilled water.

Concentration: 1 mg/mL

Buffer: Tris 0,1M, glycine 0,1M

Storage: 4 °C/-20 °C

Storage Comment: Short term storage at+4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt.

Expiry Date: 6 months

Target Details for MBD3

Target: MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))

Alternative Name: MBD3

Background: Acts as transcriptional repressor and plays a role in gene silencing. Does not bind to DNA by itself. Binds to DNA with a preference for sites containing methylated CpG dinucleotides (in vitro). Binds to a lesser degree DNA containing unmethylated CpG dinucleotides. Recruits histone deacetylases and DNA methyltransferases.

Gene ID: 53615

UniProt: O95983

Pathways: Chromatin Binding