ABCA4 antibody (C-Term)

Catalog No. ABIN4889493

The Rabbit Polyclonal anti-ABCA4 antibody (ABIN4889493) specifically detects ABCA4 in WB, ELISA and IHC. The antibody is reactive with Human, Mouse, Rat and Monkey samples.

Quick Overview for ABCA4 antibody (C-Term) (ABIN4889493)

Target: ABCA4 (ATP-Binding Cassette, Sub-Family A (ABC1), Member 4 (ABCA4))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ABCA4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-ABCA4 Antibody

Binding Specificity: C-Term

Specificity: Reacts with primate ABCA4 protein: human, mouse, rat, dog, monkeys

Cross-Reactivity (Details): ..

Purification: Purified (protein A)

Immunogen: Synthetic peptide derived from C terminal domain of human ABCA4 protein.

Application Details

Application Notes:

Working dilution: Optimal dilutions should be determined by the end user.
The following are guidelines only :
- WB : 1 to 5 μg/mL - IHC : 1/100 to 1/1000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Must be reconstituted in distilled water.

Concentration: 1 mg/mL

Buffer: Tris 0,1M, glycine 0,1M, sucrose 2 %

Storage: 4 °C/-20 °C

Storage Comment: Lyophilized powder stable for a minimum of 2 years at -20°C. Store reconstituted antibodies at +4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt.

Expiry Date: 24 months

Target Details for ABCA4

Target: ABCA4 (ATP-Binding Cassette, Sub-Family A (ABC1), Member 4 (ABCA4))

Alternative Name: Retinal-Specific ATP-Binding Cassette Transporter

Background: Defects in ABCA4 are the cause of Stargardt disease type 1 (STGD1) . In the visual cycle, acts as an inward-directed retinoid flipase, retinoid substrates imported by ABCA4 from the extracellular or intradiscal (rod) membrane surfaces to the cytoplasmic membrane surface are all-trans-retinaldehyde (ATR) and N-retinyl-phosphatidyl-ethanolamine (NR-PE). Once transported to the cytoplasmic surface, ATR is reduced to vitamin A by trans-retinol dehydrogenase (tRDH) and then transferred to the retinal pigment epithelium (RPE) where it is converted to 11-cis-retinal. May play a role in photoresponse, removing ATR/NR-PE from the extracellular photoreceptor surfaces during bleach recovery.

UniProt: P78363