NF-kB p65 antibody (C-Term)

Catalog No. ABIN4889656

There are 3+ publications for this product available. The Rabbit Polyclonal anti-NF-kB p65 antibody is suitable to detect NF-kB p65 in samples from Human. It has been validated for WB, ChIP, ChIP-seq and SSA.

Quick Overview for NF-kB p65 antibody (C-Term) (ABIN4889656)

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NF-kB p65 antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq), Supershift Assay (SSA)

Product Details anti-NF-kB p65 Antibody

Binding Specificity: C-Term

Purpose: NFkB p65 antibody (pAb)

Purification: Unpurified

Immunogen: This NFkB p65 antibody was raised against a peptide immunogen corresponding to the C-terminal of human p65.

Application Details

Application Notes: ChIP-Seq: 10 µL per ChIP ChIP: 4 µL per ChIP WB: 1:2,500 - 1:5,000 dilution

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-NF-kB p65 antibody (ABIN4889656)

Diermeier, Kolovos, Heizinger, Schwartz, Georgomanolis, Zirkel, Wedemann, Grosveld, Knoch, Merkl, Cook, Längst, Papantonis: "TNF? signalling primes chromatin for NF-?B binding and induces rapid and widespread nucleosome repositioning." in: Genome biology, Vol. 15, Issue 12, pp. 536, (2015) (PubMed).

Raaz, Schellinger, Chernogubova, Warnecke, Kayama, Penov, Hennigs, Salomons, Eken, Emrich, Zheng, Adam, Jagger, Nakagami, Toh, Toyama, Deng, Buerke, Maegdefessel, Hasenfuß, Spin, Tsao: "Transcription Factor Runx2 Promotes Aortic Fibrosis and Stiffness in Type 2 Diabetes Mellitus." in: Circulation research, Vol. 117, Issue 6, pp. 513-24, (2015) (PubMed).

Anderson, Rahmutula, Gardner: "Tumor necrosis factor-alpha inhibits endothelial nitric-oxide synthase gene promoter activity in bovine aortic endothelial cells." in: The Journal of biological chemistry, Vol. 279, Issue 2, pp. 963-9, (2004) (PubMed).

Target Details for NF-kB p65

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Alternative Name: NFkappaB p65

Background: NFkB p65 (Nuclear factor NF-kappa-B p65 subunit also known as NFkB p65 or RelA) is a member of the Rel family of transcription factors that are involved in processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. The Rel family includes c-Rel, RelA (NFkB p65, NFkB3) and RelB (I-Rel). Rel proteins form dimers with the NFkB p50 (proteolytically processed from p105/NFkB1) and p52 (proteolytically processed from p100/NFkB2) members of the NFkB family that bind DNA and activate transcription. The NFkB p65/p50 heterodimer is the most abundant form of NFkB. NFkB signaling is negatively regulated by the sequestration of the NFkB complex in the cytoplasm by its association with the IkB family of inhibitory proteins. The IkB Kinase (IKK) complex is the key enzyme involved in the activation and translocation of NFkB. The IKK complex is composed of two catalytic subunits (IKKa and IKKb) and a regulatory subunit (IKKg). Upon its activation, the IKK complex phosphorylates IkB proteins (IkBa, IkBa phospho Ser32, 36), which marks them for ubiquitination and degradation. This enables the NFkB complex to translocate to the nucleus where it regulates transcription by binding to DNA.

Molecular Weight: 65 kDa

Gene ID: 5970

NCBI Accession: NP_068810

Pathways: NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins