BAX antibody

Catalog No. ABIN4901053

This Mouse Monoclonal antibody specifically detects BAX in WB and ELISA. It exhibits reactivity toward Human and Monkey.

Quick Overview for BAX antibody (ABIN4901053)

Target: BAX (BCL2-Associated X Protein (BAX))

Reactivity: Human, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This BAX antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 2B12-G6-H6

Product Details anti-BAX Antibody

Purpose: Mouse monoclonal to Bax.

Specificity: This antibody detects endogenous levels of Bax and does not cross-react with related proteins.

Characteristics: Mouse Monoclonal to Bax.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Purified recombinant human Bax protein fragments expressed in E.coli

Isotype: IgG2a

Application Details

Application Notes: WB 1:500-2000
ELISA 1:10000-20000

Comment:

Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C, and avoid repeat freeze-thaw cycles.

Target Details for BAX

Target: BAX (BCL2-Associated X Protein (BAX))

Alternative Name: Bax

Molecular Weight: 20kDa

Gene ID: 581

UniProt: Q07812

Pathways: p53 Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response