Integrin beta 2 antibody

Catalog No. ABIN490178

The Rat Monoclonal anti-Integrin beta 2 antibody has been validated for FACS, WB, IHC, IP, IHC (fro), Func, IHC (p), BR and Neut. It is suitable to detect Integrin beta 2 in samples from Mouse.

Quick Overview for Integrin beta 2 antibody (ABIN490178)

Target: Integrin beta 2 (ITGB2)

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Integrin beta 2 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Blocking Reagent (BR), Neutralization (Neut)

Clone: M18-2

Product Details anti-Integrin beta 2 Antibody

Specificity: The rat monoclonal antibody M18/2 recognizes CD18 antigen (integrin beta2 subunit, beta2 integrin), a 95 kD type I transmembrane protein expressed on all leukocytes.

Purification: Protein G purified

Endotoxin Level: Low Endotoxin

Immunogen: Murine cytotoxic T cell glycoproteins.

Isotype: IgG2a

Application Details

Application Notes: Approved: Block, Flo, Func, IHC, IHC-Fr, IHC-P, IP, Neut, WB

Usage: Flow Cytometry: Recommended dilution - 1 μg/mL. Functional Application: Blocking or stimulation. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Azide-free PBS,  pH 7.4, 0.2 micron filter sterilized

Preservative: Azide free

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for Integrin beta 2

Target: Integrin beta 2 (ITGB2)

Alternative Name: ITGB2 / MAC-1 / CD18

Background: Name/Gene ID: ITGB2
Family: Integrin

Synonyms: ITGB2, CD18 antigen, CD18, Integrin beta chain, beta 2, LCAMB, MAC-1, MFI7, MF17, Integrin beta-2, LAD, LFA-1

Gene ID: 3689

UniProt: P05107

Pathways: NF-kappaB Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation