SAFB2 antibody (AA 848-861)

Catalog No. ABIN490534

Product Details anti-SAFB2 Antibody

Target: SAFB2 (Scaffold Attachment Factor B2 (SAFB2))

Binding Specificity: AA 848-861

Reactivity: Human, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This SAFB2 antibody is un-conjugated

Application: ELISA

Specificity: Human SAFB. This antibody is not expected to cross-react with SAFB1.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Marmoset (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-GEHNQRLEEHQARA from an internal region of human SAFB (NP_055464.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Marmoset (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:32000)

Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Preliminary experiments gave bands at approx 75  kDa and 60  kDa in lysates of HeLa cell lines after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 107  kDa according to NP_055464.1. The 75  kDa band was successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for SAFB2

Target: SAFB2 (Scaffold Attachment Factor B2 (SAFB2))

Alternative Name: SAFB2 (SAFB2 Products)

Synonyms: AA389433 antibody, AI255170 antibody, mKIAA0138 antibody, SAFB2 antibody, scaffold attachment factor B2 antibody, SAFB2 antibody, Safb2 antibody

Background: Name/Gene ID: SAFB2

Synonyms: SAFB2, KIAA0138, Scaffold attachment factor B2, SAF-B2

Gene ID: 9667

NCBI Accession: NP_055464

UniProt: Q15424