Caspase 9 antibody (AA 11-120) (PE)

Catalog No. ABIN4907432

The Rabbit Polyclonal anti-Caspase 9 antibody has been validated for WB and FACS. It is suitable to detect Caspase 9 in samples from Human, Mouse, Rat, Rabbit, Chicken and Dog.

Quick Overview for Caspase 9 antibody (AA 11-120) (PE) (ABIN4907432)

Target: Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))

Reactivity: Human, Mouse, Rat, Rabbit, Chicken, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 9 antibody is conjugated to PE

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-Caspase 9 Antibody (PE)

Binding Specificity: AA 11-120

Cross-Reactivity: Chicken, Dog, Human, Mouse, Rabbit, Rat

Predicted Reactivity: Cow

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Caspase 9 subunit p35

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Caspase 9

Target: Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))

Alternative Name: Caspase 9

Background:

Synonyms: MCH6, APAF3, APAF-3, PPP1R56, ICE-LAP6, Caspase-9, CASP-9, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, ICE-like apoptotic protease 6, CASP9

Background: Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.

Gene ID: 842

UniProt: P55211

Pathways: MAPK Signaling, RTK Signaling, Apoptosis, Caspase Cascade in Apoptosis, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Endopeptidase Activity