FHIT antibody

Catalog No. ABIN4909573

This Mouse Monoclonal antibody specifically detects FHIT in WB and FACS. It exhibits reactivity toward Human.

Quick Overview for FHIT antibody (ABIN4909573)

Target: FHIT (Fragile Histidine Triad (FHIT))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FHIT antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Clone: 3C2

Product Details anti-FHIT Antibody

Cross-Reactivity: Human

Purification: Purified by Protein G.

Immunogen: This FHIT antibody is generated from a mouse immunized with a recombinant protein.

Isotype: IgG1

Application Details

Application Notes: WB 1:300-5000
FCM 1:20-100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for FHIT

Target: FHIT (Fragile Histidine Triad (FHIT))

Alternative Name: FHIT

Background:

Synonyms: FRA3B, AP3Aase, Bis(5'-adenosyl)-triphosphatase, AP3A hydrolase, Diadenosine 5',5'''-P1,P3-triphosphate hydrolase, Dinucleosidetriphosphatase, Fragile histidine triad protein, FHIT

Background: Cleaves P(1)-P(3)-bis(5'-adenosyl) triphosphate (Ap3A) to yield AMP and ADP. Can also hydrolyze P(1)-P(4)-bis(5'-adenosyl) tetraphosphate (Ap4A), but has extremely low activity with ATP. Modulates transcriptional activation by CTNNB1 and thereby contributes to regulate the expression of genes essential for cell proliferation and survival, such as CCND1 and BIRC5. Plays a role in the induction of apoptosis via SRC and AKT1 signaling pathways. Inhibits MDM2-mediated proteasomal degradation of p53/TP53 and thereby plays a role in p53/TP53-mediated apoptosis. Induction of apoptosis depends on the ability of FHIT to bind P(1)-P(3)-bis(5'-adenosyl) triphosphate or related compounds, but does not require its catalytic activity, it may in part come from the mitochondrial form, which sensitizes the low-affinity Ca(2+) transporters, enhancing mitochondrial calcium uptake. Functions as tumor suppressor.

Gene ID: 2272

UniProt: P49789