FYN antibody

Catalog No. ABIN4909756

Product Details anti-FYN Antibody

Target: FYN (FYN Oncogene Related To SRC, FGR, YES (FYN))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FYN antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Human

Purification: Purified by Protein G.

Immunogen: This FYN antibody is generated from a mouse immunized with a recombinant preotein from human FYN.

Clone: 2C7

Isotype: IgG1

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for FYN

Target: FYN (FYN Oncogene Related To SRC, FGR, YES (FYN))

Alternative Name: FYN (FYN Products)

Synonyms: im:6908851 antibody, si:dkey-119m7.5 antibody, zgc:109996 antibody, c-fyn antibody, cfyn-a antibody, SLK antibody, SYN antibody, p59-FYN antibody, PKC antibody, fyn antibody, zgc:86720 antibody, AI448320 antibody, AW552119 antibody, FYN proto-oncogene, Src family tyrosine kinase antibody, FYN proto-oncogene, Src family tyrosine kinase S homeolog antibody, FYN proto-oncogene, Src family tyrosine kinase b antibody, FYN proto-oncogene, Src family tyrosine kinase L homeolog antibody, FYN proto-oncogene, Src family tyrosine kinase a antibody, Fyn proto-oncogene antibody, FYN antibody, fyn.S antibody, fynb antibody, fyn.L antibody, Fyn antibody, fyna antibody

Background:

Synonyms: SLK, SYN, p59-FYN, Tyrosine-protein kinase Fyn, Proto-oncogene Syn, Proto-oncogene c-Fyn, Src-like kinase, FYN

Background: Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response and axon guidance. Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain. Following activation by PKA, the protein subsequently associates with PTK2/FAK1, allowing PTK2/FAK1 phosphorylation, activation and targeting to focal adhesions. Involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta-catenin) and CTNND1 (delta-catenin). Regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubule-associated proteins MAP2 and MAPT. Promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage. Participates in signal transduction pathways that regulate the integrity of the glomerular slit diaphragm (an essential part of the glomerular filter of the kidney) by phosphorylating several slit diaphragm components including NPHS1, KIRREL and TRPC6. Plays a role in neural processes by phosphorylating DPYSL2, a multifunctional adapter protein within the central nervous system, ARHGAP32, a regulator for Rho family GTPases implicated in various neural functions, and SNCA, a small pre-synaptic protein. Participates in the downstream signaling pathways that lead to T-cell differentiation and proliferation following T-cell receptor (TCR) stimulation. Also participates in negative feedback regulation of TCR signaling through phosphorylation of PAG1, thereby promoting interaction between PAG1 and CSK and recruitment of CSK to lipid rafts. CSK maintains LCK and FYN in an inactive form. Promotes CD28-induced phosphorylation of VAV1.

Gene ID: 2534

UniProt: P06241

Pathways: JAK-STAT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Activated T Cell Proliferation, Thromboxane A2 Receptor Signaling