MBD3 antibody

Catalog No. ABIN4911809

The Mouse Monoclonal anti-MBD3 antibody is suitable to detect MBD3 in samples from Human. It has been validated for WB.

Quick Overview for MBD3 antibody (ABIN4911809)

Target: MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MBD3 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 5C3

Product Details anti-MBD3 Antibody

Cross-Reactivity: Human

Purification: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: Purified His-tagged MBD3 protein was used to produced this monoclonal antibody.

Isotype: IgG1

Application Details

Application Notes: WB 1:300-5000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for MBD3

Target: MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))

Alternative Name: MBD3

Background:

Synonyms: Methyl-CpG-binding domain protein 3, Methyl-CpG-binding protein MBD3, MBD3

Background: Acts as transcriptional repressor and plays a role in gene silencing. Does not bind to DNA by itself (PubMed:12124384). Binds to DNA with a preference for sites containing methylated CpG dinucleotides (in vitro). Binds to a lesser degree DNA containing unmethylated CpG dinucleotides (PubMed:24307175). Recruits histone deacetylases and DNA methyltransferases.

Gene ID: 53615

UniProt: O95983

Pathways: Chromatin Binding