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NCOA1 antibody (N-Term)

This Mouse Monoclonal antibody specifically detects NCOA1 in WB and EIA. It exhibits reactivity toward Human, Mouse, Rat and Dog.
Catalog No. ABIN492358

Quick Overview for NCOA1 antibody (N-Term) (ABIN492358)

Target

See all NCOA1 Antibodies
NCOA1 (Nuclear Receptor Coactivator 1 (NCOA1))

Reactivity

  • 62
  • 37
  • 17
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
Human, Mouse, Rat, Dog

Host

  • 69
  • 8
Mouse

Clonality

  • 58
  • 19
Monoclonal

Conjugate

  • 44
  • 5
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This NCOA1 antibody is un-conjugated

Application

  • 56
  • 16
  • 13
  • 13
  • 12
  • 12
  • 7
  • 6
  • 4
  • 4
  • 3
  • 3
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone

11F1
  • Binding Specificity

    • 15
    • 5
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term

    Specificity

    AM00146PU-N specifically recognizes the N-terminus of Src in Western blot at 60 kDa.

    Characteristics

    Synonyms: c-Src, pp60c-src, Proto-oncogene tyrosine-protein kinase Src, Proto-oncogene c-Src

    Purification

    Subsequent Subsequent Thiophilic and Size Exclusion Chromatography.

    Components

    incl. positive Control

    Immunogen

    synthetic peptide conjugated to KLHEpitope: N-terminus

    Isotype

    IgG1
  • Application Notes

    ELISA: Use at 0.05 μg/mLImmunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer AS00002BU-N or AS00002BU-L. Included Positive Control: Cell lysate from untreated A431 cells (See Protocols for moredetails).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Protocol

    Positive Control Provided. Cell lysate from untreated A431 cellsDescription: Cell lysate from untreated A431 cells, epidermoid carcinoma (Human)Format: Lyophilized cell lysate from serum starved A431 cells. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles.

    Restrictions

    For Research Use only
  • Reconstitution

    Restore with 1 mL H2O (15 min, RT).

    Buffer

    1 mL2 x PBS, containing 0.09 % Sodium Azide/PEG and Sucrose.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C

    Storage Comment

    For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Avoid repeated freeze / thaw cycles. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.

    Expiry Date

    12 months
  • Target

    NCOA1 (Nuclear Receptor Coactivator 1 (NCOA1))

    Alternative Name

    SRC1

    Background

    Src is the best understood member of a family of 9 tyrosine kinases regulating cellular responses to extracellular stimuli. Src is activated by PDGF and other growth factors that activate transmembrane tyrosine kinase receptors as well as increased intracellular calcium or oxidative stress. Src becomes activated upon dephosphorylation of Tyr 527, leading to a conformational change of the src molecule towards an Synonyms: Proto-oncogene c-Src, Proto-oncogene tyrosine-protein kinase Src, c-Src, pp60c-src

    Gene ID

    6714

    UniProt

    P12931

    Pathways

    Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha
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