IL-33 antibody

Catalog No. ABIN492540

The Mouse Monoclonal anti-IL-33 antibody (Clone 12H6D12) (ABIN492540) specifically detects IL-33 in WB, IHC (p) and EIA. The antibody is reactive with Human and Mouse samples.

Quick Overview for IL-33 antibody (ABIN492540)

Target: IL-33 (IL33) (Interleukin 33 (IL33))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-33 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Clone: 12H6D12

Product Details anti-IL-33 Antibody

Specificity: Recognizes Interleukin-33 (IL33).

Characteristics: Synonyms: IL-33, IL1F11, IL-1F11, NFHEV, NF-HEV, C9orf26, Interleukin-1 family member 11, Nuclear factorfrom high endothelial venules

Purification: Immunoaffinity Chromatography.

Immunogen: Recombinant IL-33 protein (Genbank accession No. NP_254274).

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: IL33 antibody can be used for detection of IL33 at 1 μg/mL. Positive Control: Recombinant IL-33 Protein. Immunohistochemistry: 5 μg/mL. Positive Control: Human lymph node tissue.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for IL-33

Target: IL-33 (IL33) (Interleukin 33 (IL33))

Alternative Name: Interleukin-33 (IL33)

Background: Interleukin-33 (IL-33) is a recently identified member of the IL-1 family of cytokines whose other members include IL-1ab, IL-1Ra and IL-18. Its receptor has been shown to be ST2, an IL-1 receptor family member that also acts as a negative regulator of TLR-IL-1R signaling and IL-1R accessory protein (IL-1RAcP). Receptor binding of IL-33 activates NF-κB and MAP kinases and induces the expression of TH2-associated cytokines such as IL-4, IL-5 and IL-6. Prolonged IL-33 treatment of mice led to the development of eosinophilia, splenomegaly, and severe pathological changes in mucosal organs such as lungs, esophagus and small intestine. Recent experiments have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. Despite its predicted molecular weight, IL-33 will often run at higher molecular weight in SDS-PAGE.Synonyms: C9orf26, IL-1F11, IL-33, IL1F11, Interleukin-1 family member 11, NF-HEV, NFHEV, Nuclear factor from high endothelial venules

Gene ID: 90865

UniProt: O95760

Pathways: Production of Molecular Mediator of Immune Response