Phospholipase C gamma 1 antibody (AA 970-990)

Catalog No. ABIN492624

This anti-Phospholipase C gamma 1 antibody is a Mouse Monoclonal antibody detecting Phospholipase C gamma 1 in WB. Suitable for Human, Mouse, Rat and Dog.

Quick Overview for Phospholipase C gamma 1 antibody (AA 970-990) (ABIN492624)

Target: Phospholipase C gamma 1 (PLCG1) (phospholipase C, gamma 1 (PLCG1))

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This Phospholipase C gamma 1 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 2D5

Product Details anti-Phospholipase C gamma 1 Antibody

Binding Specificity: AA 970-990

Specificity: This monoclonal PLCG antibody (Clone 2D5) specifically recognizes Phospholipase C gamma at 150 kDa in Western blot applications.

Characteristics: Synonyms: Phospholipase C gamma, PLC-gamma, Phosphoinositide Phospholipase C, PLC-148, PLC-II,PLC-gamma-1, PLC1, PLCG-1, EC=3.1.4.11, Phospholipase C-gamma-1

Purification: Subsequent Ultrafiltration and Size Exclusion Chromatography.

Components: incl. positive Control

Immunogen: Synthetic peptide conjugated to hemocyanin corresponding to amino acids 970-990 ofPLCgamma (Phospholipase C gamma).

Isotype: IgG1

Application Details

Application Notes: Immunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer AS00002BU-N or AS00002BU-L. Included Positive Control: Cell lysate from untreated SKOV-3 cells (See Protocols for moredetails).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Positive Control Provided. Cell lysate from untreated SKOV-3 cellsDescription: Cell lysate from untreated SKOV-3 cells, ovary adenocarcinoma (Human)Format: Lyophilized cell lysate from serum starved SKOV-3 cells. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mLPBS, containing 0.09 % Sodium Azide/PEG and Sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Avoid repeated freeze / thaw cycles. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.

Expiry Date: 12 months

Target Details for Phospholipase C gamma 1

Target: Phospholipase C gamma 1 (PLCG1) (phospholipase C, gamma 1 (PLCG1))

Alternative Name: PLCG1

Background: Phospholipase C gamma (PLCG) binds to receptor tyrosine kinases, e.g. EGFR, PDGFR, leading to phosphorylation of PLCgamma at Tyr 771, 783 and 1245. Phosphorylation of Tyr 783 leads to enzymatic activation of PLCgamma. PLC-gamma hydrolyzes phosphatidylinostitol-4,5-bisphosphate (PIP2), thus generating the second messengers inostitol-1,4,5-triphosphate (IP3) and diacylglycerol (DAG).Synonyms: EC=3.1.4.11, PLC-148, PLC-II, PLC-gamma, PLC-gamma-1, PLC1, PLCG-1, Phosphoinositide Phospholipase C, Phospholipase C gamma, Phospholipase C-gamma-1

Gene ID: 5335

UniProt: P19174

Pathways: RTK Signaling, WNT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Inositol Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Skeletal Muscle Fiber Development, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, VEGFR1 Specific Signals, VEGF Signaling