CD15 antibody

Catalog No. ABIN4949237

This anti-CD15 antibody is a Rabbit Polyclonal antibody detecting CD15 in FACS, IF and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for CD15 antibody (ABIN4949237)

Target: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD15 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CD15 Antibody

Purification: Protein A affinity

Immunogen: Purified human neutrophils were used as the immunogen for the CD15 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the CD15 antibody should be determined by the researcher.\. Flow Cytometry: 2-4 μg/million cells in 0.1ml,Immunofluorescence: 2-4 μg/mL,Immunohistochemistry (FFPE): 5-10 μg/mL for 30 min at RT

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the CD15 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for CD15

Target: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Alternative Name: CD15 / SSEA-1

Background: CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on >95 % of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.