Recombinant CFTR / Cystic Fibrosis Transmembrane Regulator (AA 258-385) antibody

Catalog No. ABIN4949317

The Rabbit Monoclonal anti-CFTR / Cystic Fibrosis Transmembrane Regulator antibody is suitable to detect CFTR / Cystic Fibrosis Transmembrane Regulator in samples from Human. It has been validated for IHC (p).

Quick Overview for Recombinant CFTR / Cystic Fibrosis Transmembrane Regulator (AA 258-385) antibody (ABIN4949317)

Target: CFTR / Cystic Fibrosis Transmembrane Regulator

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: CFTR-1775R

Product Details

Binding Specificity: AA 258-385

Purpose: Recombinant CFTR Antibody / Rabbit Monoclonal (azide and preservative free)

Purification: Protein A affinity chromatography

Immunogen: A recombinant partial protein corresponding to amino acids 258-385 from the human protein was used as the immunogen for this recombinant CFTR antibody.

Isotype: IgG, kappa

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant CFTR antibody to be titered up or down for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant CFTR antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details

Target: CFTR / Cystic Fibrosis Transmembrane Regulator

Background: Recognizes a protein of 165-170 kDa, identified as cystic fibrosis transmembrane conductance regulator (CFTR). CFTR is composed of two membrane-spanning domains (MSD), two nucleotide-binding domains (NBD), and an R domain. It is structurally similar to multidrug resistance (Mdr1) protein and both are members of the superfamily of ATP-binding cassette (ABC) transporters, also known as traffic ATPases, which are implicated in the movement of various substrates. The CFTR protein is a small conductance adenosine 3',5'-cyclic monophosphate (cAMP)-activated chloride ion channel found in the apical membranes of epithelia within the pancreas, airway, intestine, bile duct, sweat gland, and male genital ducts. CFTR is a valuable marker of human pancreatic duct cell development and differentiation.

UniProt: P13569