Topoisomerase II alpha antibody (AA 1352-1493)

Catalog No. ABIN4949922

This Mouse Monoclonal antibody specifically detects Topoisomerase II alpha in WB, IF and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for Topoisomerase II alpha antibody (AA 1352-1493) (ABIN4949922)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Topoisomerase II alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: TOP2A-1361

Product Details anti-Topoisomerase II alpha Antibody

Binding Specificity: AA 1352-1493

Purpose: Topoisomerase II alpha Antibody / TOP2A (azide and preservative free)

Purification: Protein G affinity chromatography

Immunogen: Amino acids 1352-1493 from the human protein were used as the immunogen for this Topoisomerase II alpha antibody.

Isotype: IgG2b, kappa

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Topoisomerase II alpha antibody to be titered up or down for optimal performance.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Topoisomerase II alpha antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Alternative Name: Topoisomerase II alpha

Background: This mAb recognizes an ~170 kDa protein which is identified as Topoisomerase IIa, and shows no cross-reaction with Topoisomerase IIb or I. Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIa is also implicated in drug resistance of tumor cells.

UniProt: P11388

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases