Vimentin antibody (AA 430-457)

Catalog No. ABIN3029594

The Rabbit Polyclonal anti-Vimentin antibody is suitable to detect Vimentin in samples from Human. It has been validated for WB, IHC, IF, ELISA and FACS.

Quick Overview for Vimentin antibody (AA 430-457) (ABIN3029594)

Target: Vimentin (VIM)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Vimentin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Flow Cytometry (FACS)

Product Details anti-Vimentin Antibody

Binding Specificity: AA 430-457

Purpose: Vimentin Antibody

Predicted Reactivity: Mouse,Rat,Bovine,Pig,Primate,Chicken,Hamster,Xenopus

Purification: Purified

Immunogen: A portion of amino acids 430-457 from the human protein was used as the immunogen for this Vimentin Antibody for IF / EMT Marker Antibody.

Isotype: Ig Fraction

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the Vimentin Antibody for IF / EMT Marker Antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles

Storage: -20 °C

Storage Comment: Aliquot the anti-Vimentin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for Vimentin

Target: Vimentin (VIM)

Alternative Name: Vimentin

Background: Vimentin (VIM) is a type III intermediate filament protein that serves as a central marker of mesenchymal identity and epithelial-mesenchymal transition in both normal biology and cancer progression. Vimentin Antibody for IF / EMT Marker Antibody is specifically designed for immunofluorescence-based detection of EMT-associated Vimentin expression, enabling visualization of mesenchymal phenotypes and cellular plasticity at the single-cell level. This aligns strongly with search intent such as Vimentin EMT antibody, VIM IF antibody for EMT, and EMT marker antibody for immunofluorescence. Vimentin is also referred to as Vimentin antibody, VIM antibody, and mesenchymal marker antibody in the literature, supporting strong alignment across gene, protein, and disease-focused terminology.

In immunofluorescence imaging, Vimentin is strongly upregulated during epithelial-mesenchymal transition, where epithelial cells lose polarity and cell-cell adhesion while acquiring migratory, invasive, and stem-like characteristics. The Vimentin Antibody for IF / EMT Marker Antibody enables direct visualization of this transition by highlighting cytoplasmic filamentous Vimentin expression in EMT-positive cells, often in contrast to epithelial markers such as cytokeratins. This contrast is critical for identifying transitioning cell populations within heterogeneous tumors and for mapping EMT-driven changes in cell state during cancer progression.

Vimentin expression is prominent in fibroblasts, endothelial cells, immune cells, and mesenchymal-like tumor cells, where it supports cytoskeletal remodeling, migration, and tissue invasion. In fluorescence-based workflows, the Vimentin Antibody for IF / EMT Marker Antibody is widely used to study tumor microenvironment composition, stromal activation, and EMT-associated phenotypes. It is particularly valuable in co-localization experiments, where Vimentin is analyzed alongside epithelial markers, stem cell markers, or signaling proteins to define distinct cellular states and transitional populations within complex tissues.

Functionally, Vimentin interacts with actin filaments and microtubules to regulate cell shape, adhesion, and motility, all of which are essential during EMT and metastatic progression. Its dynamic reorganization through phosphorylation supports cytoskeletal flexibility required for invasion and migration. The Vimentin Antibody for IF / EMT Marker Antibody differentiator is central to this antibody, positioning it specifically for fluorescence imaging studies of tumor plasticity, invasive behavior, and epithelial-to-mesenchymal transition. A rabbit polyclonal Vimentin Antibody for IF provides strong fluorescent signal and enables sensitive detection of Vimentin / VIM expression in EMT-active cell populations, supporting advanced cancer biology and cell state research.

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis