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Vimentin antibody (AA 430-457)

Name: Vimentin antibody (AA 430-457)
SKU: ABIN3029594
Price: 606.92 USD
Availability: InStock

VIM Reactivity: Human WB, IHC, ELISA, IF, FACS Host: Rabbit Polyclonal unconjugated

Western blot analysis of anti-Vimentin antibody and NCI-H460 lysate (Vimentin antibody (AA 430-457))

Anti-Vimentin antibody flow cytometric analysis of HeLa cells (right histogram) compared to a negative control cell (left histogram). (Vimentin antibody (AA 430-457))

FACS

Anti-Vimentin antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue. (Vimentin antibody (AA 430-457))

IHC

Fluorescent confocal image of SY5Y cells stained with anti-Vimentin antibody at 1:100. (Vimentin antibody (AA 430-457))

IHC

FACS

7 images

Catalog No. ABIN3029594

Datasheet as PDF

Target See all Vimentin (VIM) Antibodies

Vimentin (VIM)
Binding Specificity
32

18

16

16

16

12

11

8

7

7

4

3

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 430-457
Reactivity
322

125

122

60

57

42

41

35

29

21

20

13

13

8

7

6

6

3

3

2

1

1

1

1

1

1

1
Human
Host
183

122

7

7

6

4

2
Rabbit
Compatible Secondaries
Clonality
196

134
Polyclonal
Conjugate
187

19

14

13

10

7

7

7

7

7

7

7

4

4

4

4

4

3

2

2

2

2

2

2

2

1

1
This Vimentin antibody is un-conjugated
Application
270

127

96

95

91

56

43

40

39

28

23

20

8

5

4

4

4

2

2

2

2

1

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Cross-Reactivity (Details)

Expected species reactivity: Bovine, Chicken, Hamster, Mouse, Pig, Primate, Rat, Xenopus

Purification

Purified

Immunogen

A portion of amino acids 430-457 from the human protein was used as the immunogen for this anti-Vimentin antibody.

Isotype

Ig Fraction

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VIM Reactivity: Human, Mouse, Rat, Pig, Chicken WB, IHC (p), ICC, IP Host: Mouse Monoclonal VI-10 unconjugated

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VIM Reactivity: Human, Mouse WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB41034 unconjugated

anti-Vimentin (VIM) (AA 1-200) antibody
VIM Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human WB, IHC, ICC, IP Host: Mouse Monoclonal C8 unconjugated

anti-Vimentin (VIM) (AA 430-457), (C-Term) antibody
VIM Reactivity: Human WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB16691 unconjugated

anti-Vimentin (VIM) (AA 2-466) antibody
VIM Reactivity: Human, Mouse, Rat WB, IHC, ELISA, FACS Host: Mouse Monoclonal 3A1F2 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human WB, IHC, StM Host: Rabbit Monoclonal VIM-1937R unconjugated Recombinant Antibody

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the anti-Vimentin antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Immunofluorescence: 1:100,Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50

Restrictions

For Research Use only
Format

Liquid

Buffer

In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Aliquot the anti-Vimentin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Target

Vimentin (VIM)

Alternative Name

Vimentin (VIM Products)

Synonyms

CTRCT30 antibody, cb28 antibody, vime antibody, vim antibody, vim1 antibody, vim2 antibody, VIM antibody, Vimentin antibody, vim4 antibody, vimentin antibody, vimentin L homeolog antibody, vimentin S homeolog antibody, VIM antibody, Vim antibody, vim antibody, vim.L antibody, vim.S antibody

Background

Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.

UniProt

P08670

Pathways

Caspase Cascade in Apoptosis