EIF2AK2 antibody (AA 519-550)

Catalog No. ABIN3030885

This anti-EIF2AK2 antibody is a Rabbit Polyclonal antibody detecting EIF2AK2 in WB, IHC and ELISA. Suitable for Human and Mouse.

Quick Overview for EIF2AK2 antibody (AA 519-550) (ABIN3030885)

Target: EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF2AK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-EIF2AK2 Antibody

Binding Specificity: AA 519-550

Purification: Purified

Immunogen: A portion of amino acids 519-550 from the human protein was used as the immunogen for this PRKR antibody.

Isotype: Ig Fraction

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the PRKR antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the PRKR antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for EIF2AK2

Target: EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))

Alternative Name: PRKR

Background: Interferon-induced, double-stranded RNA-activated protein kinase is a serine-threonine kinase. Activation by dsRNAs leads to autophosphorylation of PRKR and allows the kinase to phosphorylate its natural substrate, the alpha subunit of eukaryotic protein synthesis initiation factor-2 (EIF2-alpha), leading to the inhibition of protein synthesis. Human gamma-interferon (IFNG) mRNA exploits localized activation of PRKR in the cell to regulate its own translation. IFNG mRNA activates PRKR through a pseudoknot in its 5-prime untranslated region. The HCV envelope protein E2 contains a sequence identical with phosphorylation sites of the interferon-inducible protein kinase PRKR and the translation initiation factor EIF2-alpha, a target of PRKR. E2 inhibits the kinase activity of PRKR and blocks its inhibitory effect on protein synthesis and cell growth, which provides one mechanism by which HCV may circumvent the antiviral effect of interferon.

UniProt: P19525

Pathways: DNA Damage Repair, ER-Nucleus Signaling, Hepatitis C