AIF antibody

Catalog No. ABIN4953229

The Mouse Monoclonal anti-AIF antibody is suitable to detect AIF in samples from Human. It has been validated for WB, IF and ICC.

Quick Overview for AIF antibody (ABIN4953229)

Target: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This AIF antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 8H1-B10-A12

Product Details anti-AIF Antibody

Purification: Protein G affinity

Immunogen: A human recombinant partial protein was used as the immunogen for this AIF antibody.

Isotype: IgG2a

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the AIF antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,ICC/IF: 1:200

Restrictions: For Research Use only

Handling

Buffer: Antibody in PBS with 50 % Glycerol and 0.03 % ProClin 300

Storage: -20 °C

Storage Comment: Store the AIF antibody at -20°C.

Target Details for AIF

Target: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Alternative Name: AIFM1 / AIF / Apoptosis-Inducing Factor

Background: Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner. [UniProt]

UniProt: O95831

Pathways: Apoptosis, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Warburg Effect