Recombinant HIF1A antibody

Catalog No. ABIN4953357

Product Details anti-HIF1A Antibody

Target: HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This HIF1A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Characteristics: This recombinant HIF1A antibody reacts to human Hypoxia-inducible factor 1-alpha.

Purification: Protein A purified from animal origin-free supernatant

Immunogen: A peptide corresponding to Hypoxia-inducible factor 1-alpha was used as the immunogen for this recombinant HIF1A antibody.

Clone: RM242

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the recombinant HIF1A antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH 6 Citrate buffer or pH 9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.\. IHC (FFPE): 1:500-1:1000 (1),Western Blot: 1:1000-1:2000

Restrictions: For Research Use only

Handling

Buffer: Antibody in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the recombinant HIF1A antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol).

Target Details for HIF1A

Target: HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Alternative Name: HIF1A / HIF-1 alpha (HIF1A Products)

Background: This recombinant HIF1A antibody reacts to human Hypoxia-inducible factor 1-alpha.

Gene ID: 3091

UniProt: Q16665

Pathways: Positive Regulation of Peptide Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Transition Metal Ion Homeostasis, Tube Formation, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals, Warburg Effect