This recombinant Histone H2AX antibody reacts to Histone H2AX protein, independent of post-translational modifications. No cross reactivity with other histone proteins.
Purification
Protein A purified from animal origin-free supernatant
Immunogen
A peptide corresponding to the C-terminus of human Histone H2AX was used as the immunogen for this recombinant Histone H2AX antibody.
The stated application concentrations are suggested starting points. Titration of the recombinant Histone H2AX antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western Blot: 0.5-2 μg/mL,Immunocytochemistry: 1-2 μg/mL,ELISA: 0.2-1 μg/mL
Restrictions
For Research Use only
Buffer
1 mg/mL in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Store the recombinant Histone H2AX antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol).
Target
H2AFX
(H2A Histone Family, Member X (H2AFX))
Alternative Name
Histone H2AX
Background
This recombinant Histone H2AX antibody reacts to Histone H2AX protein, independent of post-translational modifications. No cross reactivity with other histone proteins.