This recombinant Histone H2AX antibody reacts to Histone H2AX protein, independent of post-translational modifications. No cross reactivity with other histone proteins.
Purification
Protein A purified from animal origin-free supernatant
Immunogen
A peptide corresponding to the C-terminus of human Histone H2AX was used as the immunogen for this recombinant Histone H2AX antibody.
H2AFX
Reactivity: Human
IHC, ELISA
Host: Rabbit
Polyclonal
unconjugated
Application Notes
The stated application concentrations are suggested starting points. Titration of the recombinant Histone H2AX antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western Blot: 0.5-2 μg/mL,Immunocytochemistry: 1-2 μg/mL,ELISA: 0.2-1 μg/mL
Restrictions
For Research Use only
Buffer
1 mg/mL in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Store the recombinant Histone H2AX antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol).
Target
H2AFX
(H2A Histone Family, Member X (H2AFX))
Alternative Name
Histone H2AX
Background
This recombinant Histone H2AX antibody reacts to Histone H2AX protein, independent of post-translational modifications. No cross reactivity with other histone proteins.