AKT2 antibody (AA 452-481)

Catalog No. ABIN3029471

The Rabbit Polyclonal anti-AKT2 antibody has been validated for WB, IHC, ELISA, IF. It is suitable to detect AKT2 in samples from Human.

Quick Overview for AKT2 antibody (AA 452-481) (ABIN3029471)

Target: AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AKT2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-AKT2 Antibody

Binding Specificity: AA 452-481

Cross-Reactivity (Details): Expected species reactivity: Mouse, Rat

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 452-481 from the human protein was used as the immunogen for this AKT2 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the AKT2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Immunofluorescence: 1:10-1:50,Western blot: 1:1000,IHC (Paraffin): 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the AKT2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for AKT2

Target: AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))

Alternative Name: AKT2

Background: AKT2 is a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. AKT2 has been shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression of AKT2 contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. AKT2 is a general protein kinase capable of phophorylating several known proteins.

UniProt: P31751

Pathways: PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, TLR Signaling, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling