Aurora A antibody

Catalog No. ABIN3030072

The Mouse Monoclonal anti-Aurora A antibody (Clone 1364CT291-108-155) (ABIN3030072) specifically detects Aurora A in WB, ELISA and IHC. The antibody is reactive with Human samples.

Quick Overview for Aurora A antibody (ABIN3030072)

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Aurora A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone: 1364CT291-108-155

Product Details anti-Aurora A Antibody

Purification: Purified

Immunogen: This AURKA antibody was produced from a mouse immunized with a KLH conjugated synthetic peptide between amino acids from the human region of human AURKA.

Isotype: IgG1 kappa

Application Details

Application Notes: Titration of the AURKA antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. IHC (Paraffin): 1:25,Western blot: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the AURKA antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for Aurora A

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Alternative Name: AURKA

Background: Mitotic serine/threonine kinases that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis. Required for initial activation of CDK1 at centrosomes. Phosphorylates numerous target proteins, including ARHGEF2, BORA, BRCA1, CDC25B, DLGP5, HDAC6, KIF2A, LATS2, NDEL1, PARD3, PPP1R2, PLK1, RASSF1, TACC3, p53/TP53 and TPX2. Regulates KIF2A tubulin depolymerase activity. Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization. Also acts as a key regulatory component of the p53/TP53 pathway, and particularly the checkpoint-response pathways critical for oncogenic transformation of cells, by phosphorylating and stabilizing p53/TP53. Phosphorylates its own inhibitors, the protein phosphatase type 1 (PP1) isoforms, to inhibit their activity. Necessary for proper cilia disassembly prior to mitosis.

UniProt: O14965

Pathways: Cell Division Cycle, Asymmetric Protein Localization