BAX antibody (AA 41-76)

Catalog No. ABIN3030145

This Rabbit Polyclonal antibody specifically detects BAX in WB, IHC, ELISA and FACS. It exhibits reactivity toward Human.

Quick Overview for BAX antibody (AA 41-76) (ABIN3030145)

Target: BAX (BCL2-Associated X Protein (BAX))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAX antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Product Details anti-BAX Antibody

Binding Specificity: AA 41-76

Cross-Reactivity (Details): Expected species reactivity: Bovine

Purification: Purified

Immunogen: A portion of amino acids 41-76 (BH3 domain) from the human protein was used as the immunogen for this Bax antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the Bax antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Bax antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for BAX

Target: BAX (BCL2-Associated X Protein (BAX))

Alternative Name: Bax

Background: Bax belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. Bax forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of the Bax gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis.

UniProt: Q07812

Pathways: p53 Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response