The Rabbit Polyclonal anti-KIT antibody has been validated for WB, FACS, ELISA and IHC. It is suitable to detect KIT in samples from Human and Primate.
Titration of the cKit antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50
Restrictions
For Research Use only
Format
Liquid
Buffer
In 1X PBS pH 7.4 with 0.09 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Aliquot the cKit antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Target
KIT
(Mast/stem Cell Growth Factor Receptor (KIT))
Alternative Name
cKit
Background
C-Kit is a Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. [UniProt]