E2F1 antibody (AA 310-344)

Catalog No. ABIN3030785

The Rabbit Polyclonal anti-E2F1 antibody is suitable to detect E2F1 in samples from Human. It has been validated for WB and ELISA.

Quick Overview for E2F1 antibody (AA 310-344) (ABIN3030785)

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-E2F1 Antibody

Binding Specificity: AA 310-344

Purpose: E2F1 Antibody

Purification: Antigen affinity

Immunogen: A portion of amino acids 310-344 from the human protein was used as the immunogen for this E2F1 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the E2F1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the E2F1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for E2F1

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Alternative Name: E2F1

Background: E2F1 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another two members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.

UniProt: Q01094

Pathways: p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy