Glypican 3 antibody (AA 529-560)

Catalog No. ABIN3031100

The Rabbit Polyclonal anti-Glypican 3 antibody has been validated for IF, WB and ELISA. It is suitable to detect Glypican 3 in samples from Human and Mouse.

Quick Overview for Glypican 3 antibody (AA 529-560) (ABIN3031100)

Target: Glypican 3 (GPC3)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Glypican 3 antibody is un-conjugated

Application: Immunofluorescence (IF), Western Blotting (WB), ELISA

Product Details anti-Glypican 3 Antibody

Binding Specificity: AA 529-560

Purification: Purified

Immunogen: A portion of amino acids 529-560 from the human protein was used as the immunogen for this GPC3 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the GPC3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Immunofluorescence: 1:10-1:50,Western blot: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the GPC3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for Glypican 3

Target: Glypican 3 (GPC3)

Alternative Name: GPC3

Background: GPC3 is a cell surface proteoglycan that bears heparan sulfate. This protein may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. Members of the glypican-related integral membrane proteoglycan family contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol (GPI) linkage. These proteins may play a role in the control of cell division, growth regulation, and tumor predisposition. Deletion mutations in GPC3 are the cause of Simpson-Golabi-Behmel syndrome (SGBS), also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies.

UniProt: P51654

Pathways: Glycosaminoglycan Metabolic Process