c-MET antibody (Catalytic Domain)

Catalog No. ABIN3031757

The Mouse Monoclonal anti-c-MET antibody has been validated for WB, ELISA, IHC and IF. It is suitable to detect c-MET in samples from Human and Mouse.

Quick Overview for c-MET antibody (Catalytic Domain) (ABIN3031757)

Target: c-MET (MET) (Met Proto-Oncogene (MET))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This c-MET antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: 4AT44

Product Details anti-c-MET Antibody

Binding Specificity: Catalytic Domain

Purification: Purified

Immunogen: This MET antibody was produced from mice immunized with purified recombinant protein encoding the catalytic domain of human MET/HGFR.

Isotype: IgG1

Application Details

Application Notes: Titration of the MET antibody (HGFR) may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,Immunofluorescence: 1:100,IHC (Paraffin): 1:25-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the MET antibody (HGFR) and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for c-MET

Target: c-MET (MET) (Met Proto-Oncogene (MET))

Alternative Name: MET (HGFR)

Background: The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene.

UniProt: P08581

Pathways: RTK Signaling, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor