MGMT antibody (AA 156-182)

Catalog No. ABIN3032088

The Rabbit Polyclonal anti-MGMT antibody (ABIN3032088) specifically detects MGMT in WB, IHC, ELISA and FACS. The antibody is reactive with Human samples.

Quick Overview for MGMT antibody (AA 156-182) (ABIN3032088)

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MGMT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Product Details anti-MGMT Antibody

Binding Specificity: AA 156-182

Purification: Purified

Immunogen: A portion of amino acids 156-182 from the human protein was used as the immunogen for this MGMT antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the MGMT antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the MGMT antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for MGMT

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Alternative Name: MGMT

Background: MGMT is involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. This protein repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the protein is irreversibly inactivated.

UniProt: P16455

Pathways: DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus