MYLK3 antibody (AA 40-69)

Catalog No. ABIN3031919

Product Details anti-MYLK3 Antibody

Target: MYLK3 (Myosin Light Chain Kinase 3 (MYLK3))

Binding Specificity: AA 40-69

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MYLK3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Purification: Purified

Immunogen: A portion of amino acids 40-69 from the human protein was used as the immunogen for this MYLK3 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the MYLK3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the MYLK3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for MYLK3

Target: MYLK3 (Myosin Light Chain Kinase 3 (MYLK3))

Alternative Name: MYLK3 (MYLK3 Products)

Synonyms: MLCK antibody, MLCK2 antibody, caMLCK antibody, D830007F02Rik antibody, RGD1305801 antibody, myosin light chain kinase 3 antibody, MYLK3 antibody, Mylk3 antibody

Background: MLCK/MYLK3, a member of the Ser/Thr protein kinase family, is a calcium/calmodulin-dependent enzyme responsible for smooth muscle contraction via phosphorylation of a specific serine in the N-terminus of myosin light chains (MLC), an event that facilitates myosin interaction with actin filaments. It is a central determinant in the development of vascular permeability and tissue edema formation. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. MLCK acts as a critical participant in signaling sequences that result in fibroblast apoptosis. Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. The Telokin isoform, which binds calmodulin, has been found in a wide variety of adult and fetal tissues. MLCK is probably down-regulated by phosphorylation. The protein contains 1 fibronectin type III domain and 9 immunoglobulin-like C2-type domains.

UniProt: Q32MK0

Pathways: Regulation of Muscle Cell Differentiation