ELANE antibody (AA 13-39)

Catalog No. ABIN3030798

Product Details anti-ELANE Antibody

Target: ELANE (Elastase, Neutrophil Expressed (ELANE))

Binding Specificity: AA 13-39

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ELANE antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 13-39 from the human protein was used as the immunogen for this Neutrophil elastase antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the Neutrophil elastase antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Neutrophil elastase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for ELANE

Target: ELANE (Elastase, Neutrophil Expressed (ELANE))

Alternative Name: Neutrophil Elastase (ELANE Products)

Synonyms: ELA2 antibody, GE antibody, HLE antibody, HNE antibody, NE antibody, PMN-E antibody, SCN1 antibody, Ela2 antibody, F430011M15Rik antibody, ela2 antibody, ELANE antibody, elastase, neutrophil expressed antibody, proteinase 3 antibody, ELANE antibody, Elane antibody, prtn3 antibody

Background: Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins. The product of this gene hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils. The enzyme may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. This protein degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is clustered with other serine protease gene family members, azurocidin 1 and proteinase 3 genes, at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation.

UniProt: P08246