Osteopontin antibody (AA 14-40)

Catalog No. ABIN3032521

This Rabbit Polyclonal antibody specifically detects Osteopontin in WB, ELISA, IHC and IF. It exhibits reactivity toward Human.

Quick Overview for Osteopontin antibody (AA 14-40) (ABIN3032521)

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Osteopontin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Osteopontin Antibody

Binding Specificity: AA 14-40

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 14-40 from the human protein was used as the immunogen for this Osteopontin antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the Osteopontin antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Immunofluorescence: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Osteopontin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for Osteopontin

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Alternative Name: Osteopontin

Background: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity (By similarity).

UniProt: P10451

Pathways: Regulation of Cell Size