BRAF antibody (pSer445)

Catalog No. ABIN3029547

This anti-BRAF antibody is a Rabbit Polyclonal antibody detecting BRAF in ELISA and DB. Suitable for Human.

Quick Overview for BRAF antibody (pSer445) (ABIN3029547)

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRAF antibody is un-conjugated

Application: ELISA, Dot Blot (DB)

Product Details anti-BRAF Antibody

Binding Specificity: pSer445

Cross-Reactivity (Details): Expected species reactivity: Mouse, Chicken

Purification: Antigen affinity purified

Immunogen: This phospho-BRAF antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS445 of human BRAF.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the phospho-BRAF antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Dot blot: 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the phospho-BRAF antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for BRAF

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Alternative Name: BRAF

Background: BRAF is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It may play a role in the postsynaptic responses of hippocampal neuron. Defects in BRAF are a cause of cardiofaciocutaneous syndrome (CFC syndrome), and a wide range of cancers such as lung cancer, non-Hodgkins lymphoma, and colorectal cancer.

UniProt: P15056

Pathways: MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy