ErbB2/Her2 antibody (pTyr1222)

Catalog No. ABIN3029506

This Rabbit Polyclonal antibody specifically detects ErbB2/Her2 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for ErbB2/Her2 antibody (pTyr1222) (ABIN3029506)

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ErbB2/Her2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-ErbB2/Her2 Antibody

Binding Specificity: pTyr1222

Purpose: HER2 Antibody / ErbB2 (pY1222)

Purification: Antigen affinity purified

Immunogen: This phospho-ErbB2 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pY1222 of the human protein.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the Phospho-ErbB2 (pY1222) Antibody / Signal Propagation Marker may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles

Storage: -20 °C

Storage Comment: Aliquot the phospho-ErbB2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for ErbB2/Her2

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Alternative Name: Erbb2

Background: ErbB2 receptor tyrosine kinase 2 (ERBB2), also known as HER2, is regulated by phosphorylation at multiple intracellular residues that control signaling intensity, duration, and downstream pathway activation. Phospho-ErbB2 (pY1222) antibody, also referred to as phospho-HER2 antibody and phospho-ERBB2 antibody in the literature, detects phosphorylation at tyrosine 1222, a site associated with continued signaling following receptor activation. For evaluation of initial adaptor recruitment, see our < a href=../tds/phospho-erbb2-antibody-py1221-f52419>Phospho-ErbB2 (pY1221) antibody.

Phosphorylation at Y1222 occurs within a signaling motif adjacent to Y1221, forming part of a cluster that recruits adaptor proteins and supports downstream signaling. While initial phosphorylation events promote recruitment of signaling complexes, phosphorylation at Y1222 contributes to maintenance and amplification of signaling through pathways such as PI3K/AKT and MAPK. This positions Y1222 as an indicator of ongoing receptor activity and signal propagation.

HER2 signaling is a major driver of tumor biology, particularly in cancers characterized by ERBB2 amplification. In these settings, phosphorylation at Y1222 contributes to sustained activation of signaling pathways that promote tumor cell survival, proliferation, and resistance to stress. Detection of this phosphorylation site provides insight into the persistence of signaling activity within tumor cells rather than early activation alone.

Y1222 functions as part of a coordinated phosphorylation network that includes nearby residues such as Y1221 and Y1248. Together, these sites regulate the progression of signaling from initiation to amplification and sustained activity. The ability to detect phosphorylation at Y1222 complements analysis of other sites and provides a more complete view of HER2 signaling dynamics across different stages.

Because phosphorylation at Y1222 reflects ongoing signaling, it is particularly useful for studying sustained pathway activation and signal maintenance in experimental systems. This includes models of chronic receptor activation, tumor progression, and response to targeted therapies.

Phospho-specific detection of ERBB2 at Y1222 enables selective analysis of receptor activity during later stages of signaling. This supports studies focused on pathway propagation and signal persistence.

For detection of activated HER2 phosphorylation, see our < a href=../tds/p-erbb2-antibody-py1248-f48395>HER2 phospho antibody (pY1248).

UniProt: P04626

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development