ErbB2/Her2 antibody (pTyr1140)

Catalog No. ABIN3029502

The Rabbit Polyclonal anti-ErbB2/Her2 antibody (ABIN3029502) specifically detects ErbB2/Her2 in WB, ELISA and DB. The antibody is reactive with Human and Mouse samples.

Quick Overview for ErbB2/Her2 antibody (pTyr1140) (ABIN3029502)

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ErbB2/Her2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Dot Blot (DB)

Product Details anti-ErbB2/Her2 Antibody

Binding Specificity: pTyr1140

Purpose: HER2 Antibody / ErbB2 (pY1140)

Purification: Antigen affinity purified

Immunogen: This phospho-ErbB2 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pY1140 of mouse HER2/ERBB2.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the Phospho-ErbB2 (pY1140) Antibody / Signaling Marker may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles

Storage: -20 °C

Storage Comment: Aliquot the phospho-ErbB2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for ErbB2/Her2

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Alternative Name: Erbb2

Background: ErbB2 receptor tyrosine kinase 2 (ERBB2), also known as HER2, is a transmembrane receptor that regulates cell proliferation, survival, and differentiation through activation of intracellular signaling pathways. Phospho-ErbB2 (pY1140) antibody, also referred to as phospho-HER2 antibody and phospho-ERBB2 antibody in the literature, detects phosphorylation at tyrosine 1140, a residue located within the intracellular domain of HER2 that contributes to downstream signaling activity.

Phosphorylation of HER2 occurs at multiple tyrosine residues following receptor activation, forming a coordinated signaling network that governs pathway engagement. Y1140 is positioned within this network and participates in transmission of signals to downstream pathways such as PI3K/AKT and MAPK. While not as extensively characterized as major activation sites, phosphorylation at Y1140 reflects receptor signaling activity and contributes to propagation of intracellular responses following receptor engagement.

HER2 signaling is a major driver of tumor biology, particularly in cancers characterized by ERBB2 amplification such as breast carcinoma. In these settings, phosphorylation at residues including Y1140 is associated with active signaling states that support tumor cell growth and survival. Detection of phosphorylation at this site provides insight into receptor activity within the broader signaling cascade rather than serving as a primary activation marker alone.

Y1140 functions within a phosphorylation cluster that includes nearby residues such as Y1112, Y1139, Y1221, Y1222, and Y1248. Together, these sites regulate the initiation, propagation, and integration of HER2-mediated signaling. Analysis of phosphorylation at Y1140 complements detection of these other residues and supports a more comprehensive understanding of HER2 signaling dynamics.

Phospho-specific detection of ERBB2 at Y1140 enables investigation of receptor signaling beyond primary activation events, supporting studies focused on pathway activity, signal propagation, and cellular response mechanisms in both normal and disease contexts.

For detection of activated HER2 phosphorylation, see our < a href=../tds/p-erbb2-antibody-py1248-f48395>HER2 phospho antibody (pY1248).

UniProt: P70424

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development