RPS6KB1 antibody (pSer418)

Catalog No. ABIN3032482

This Rabbit Polyclonal antibody specifically detects RPS6KB1 in ELISA and DB. It exhibits reactivity toward Human.

Quick Overview for RPS6KB1 antibody (pSer418) (ABIN3032482)

Target: RPS6KB1 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPS6KB1 antibody is un-conjugated

Application: ELISA, Dot Blot (DB)

Product Details anti-RPS6KB1 Antibody

Binding Specificity: pSer418

Purpose: Phospho-S6K Antibody [p-p70S6K) (pS418)

Predicted Reactivity: Bovine,Mouse,Rabbit,Rat

Purification: Antigen affinity

Immunogen: This phospho-S6K antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS418 of human RPS6KB1.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the phospho-S6K antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles

Storage: -20 °C

Storage Comment: Aliquot the phospho-S6K antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for RPS6KB1

Target: RPS6KB1 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))

Alternative Name: S6K

Background: P70 S6K is a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding this gene and overexpression of this kinase are seen in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized.

UniProt: P23443

Pathways: PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Regulation of Cell Size, Skeletal Muscle Fiber Development, Feeding Behaviour, G-protein mediated Events, Smooth Muscle Cell Migration, Interaction of EGFR with phospholipase C-gamma, Warburg Effect