PUMA antibody (AA 119-154)

Catalog No. ABIN3030112

This Rabbit Polyclonal antibody specifically detects PUMA in WB, ELISA, IHC, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for PUMA antibody (AA 119-154) (ABIN3030112)

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PUMA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-PUMA Antibody

Binding Specificity: AA 119-154

Cross-Reactivity (Details): Expected species reactivity: Mouse, Rat

Purification: Purified

Immunogen: A portion of amino acids 119-154 (BH3 domain) from the human protein was used as the immunogen for this Puma antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the Puma antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Puma antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for PUMA

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Alternative Name: Puma

Background: PUMA is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.

UniProt: Q9BXH1

Pathways: p53 Signaling, Positive Regulation of Endopeptidase Activity