UHRF1 antibody (AA 229-257)

Catalog No. ABIN3029440

This Rabbit Polyclonal antibody specifically detects UHRF1 in WB, ELISA, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for UHRF1 antibody (AA 229-257) (ABIN3029440)

Target: UHRF1 (Ubiquitin-Like, Containing PHD and RING Finger Domains, 1 (UHRF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UHRF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-UHRF1 Antibody

Binding Specificity: AA 229-257

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 229-257 from the human protein was used as the immunogen for this UHRF1 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the UHRF1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,Flow Cytometry: 1:10-1:50,Immunofluorescence: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the UHRF1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for UHRF1

Target: UHRF1 (Ubiquitin-Like, Containing PHD and RING Finger Domains, 1 (UHRF1))

Alternative Name: UHRF1

Background: UHRF1 is a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone acetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint.

UniProt: Q96T88