Vimentin antibody (AA 63-90)

Catalog No. ABIN3029590

This Rabbit Polyclonal antibody specifically detects Vimentin in WB, IHC, IF and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for Vimentin antibody (AA 63-90) (ABIN3029590)

Target: Vimentin (VIM)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Vimentin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Product Details anti-Vimentin Antibody

Binding Specificity: AA 63-90

Purpose: Vimentin Antibody

Predicted Reactivity: Rat,Bovine,Primate,Hamster

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 63-90 from the human protein was used as the immunogen for this Vimentin Antibody for IF / Cytoskeletal Filament Marker Antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the Vimentin Antibody for IF / Cytoskeletal Filament Marker Antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles

Storage: -20 °C

Storage Comment: Aliquot the Vimentin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for Vimentin

Target: Vimentin (VIM)

Alternative Name: Vimentin

Background: Vimentin (VIM) is a type III intermediate filament protein that forms a highly organized cytoplasmic filament network in mesenchymal cells, where it maintains structural integrity and regulates cellular morphology. Vimentin Antibody for IF / Cytoskeletal Filament Marker Antibody is specifically optimized for immunofluorescence-based visualization of Vimentin filament architecture, enabling high-resolution imaging of cytoskeletal networks in fixed and permeabilized cells. This aligns strongly with search intent such as Vimentin immunofluorescence antibody, VIM IF antibody, and cytoskeletal filament marker antibody. Vimentin is also referred to as Vimentin antibody, VIM antibody, and intermediate filament marker antibody in the literature, supporting strong alignment between gene, protein, and imaging-focused terminology.

In immunofluorescence and confocal microscopy applications, Vimentin forms a distinctive filamentous network that extends from the perinuclear region outward to the cell periphery. The Vimentin Antibody for IF / Cytoskeletal Filament Marker Antibody highlights these elongated cytoplasmic fibers, allowing researchers to directly visualize filament organization, network density, and structural remodeling within individual cells. This filament-specific staining pattern is a defining feature of the cytoskeletal filament marker antibody positioning and is essential for studies examining cytoskeletal architecture, cell polarity, and mechanical stability.

Vimentin is widely expressed in fibroblasts, endothelial cells, leukocytes, and other mesenchymal-derived populations, where its filament network supports migration, adhesion, and intracellular transport. In fluorescence imaging workflows, the Vimentin Antibody for IF / Cytoskeletal Filament Marker Antibody enables detailed assessment of cytoskeletal rearrangement during processes such as cell spreading, migration, and epithelial-mesenchymal transition. It is also commonly used in co-localization studies alongside actin, tubulin, or organelle markers to evaluate spatial relationships between cytoskeletal systems.

Functionally, Vimentin interacts with actin filaments and microtubules to coordinate cytoskeletal dynamics and intracellular organization. Its assembly and disassembly are regulated through phosphorylation and other post-translational modifications, which drive filament reorganization during mitosis and stress responses. The Vimentin Antibody for IF / Cytoskeletal Filament Marker Antibody differentiator is central to this antibody, positioning it specifically for fluorescence imaging applications where visualization of filament structure, network continuity, and subcellular distribution is critical. A rabbit polyclonal Vimentin Antibody for IF provides strong fluorescent signal and clear filament resolution, supporting high-quality imaging of Vimentin / VIM cytoskeletal networks in single-cell and multi-cellular systems.

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis