PPM1K antibody (N-Term)

Catalog No. ABIN497953

Product Details anti-PPM1K Antibody

Target: PPM1K (Protein Phosphatase, Mg2+/Mn2+ Dependent, 1K (PPM1K))

Binding Specificity: AA 1-324, N-Term

Reactivity: Human, Mouse, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPM1K antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Recognizes Protein phosphatase 1K/PPM1K.
Cellular Localization: Mitochondrion matrix.

Cross-Reactivity (Details): Species reactivity (expected):Mouse (92 %), Bovine (93 %).
Species reactivity (tested):Human.

Purification: Antigen-Affinity Chromatography.

Immunogen: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 324 of Human PPM1K

Isotype: IgG

Application Details

Application Notes: Western blotting: 1/500-1/3000. Positive Control: 293T. Immunohistochemistry on Formalin-Fixed Paraffin-Embedded Sections: 1/100-1/500. Antigen retrieval using heat mediated 10 mM Citrate buffer or Tris-EDTA buffer PH8.0 isSuggested.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 0.1 M Tris, 0.1 M Glycine, 10 % Glycerol and 0.01 % Thimerosal as preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at -20 °C.

Target Details for PPM1K

Target: PPM1K (Protein Phosphatase, Mg2+/Mn2+ Dependent, 1K (PPM1K))

Abstract: PPM1K Products

Synonyms: 2900063A19Rik antibody, A930026L03Rik antibody, PP2Cm antibody, im:6912645 antibody, zgc:113207 antibody, BDP antibody, MSUDMV antibody, PP2Ckappa antibody, PTMP antibody, UG0882E07 antibody, RGD1308501 antibody, protein phosphatase 1K (PP2C domain containing) antibody, protein phosphatase, Mg2+/Mn2+ dependent, 1K antibody, protein phosphatase, Mg2+/Mn2+ dependent 1K antibody, protein phosphatase, Mg2+/Mn2+ dependent 1K S homeolog antibody, Ppm1k antibody, ppm1k antibody, PPM1K antibody, ppm1k.S antibody

Background: Regulates the mitochondrial permeability transition pore and is essential for cellular survival and development.Synonyms: PP2C domain-containing protein phosphatase 1K, PP2C-kappa, PP2C-like mitochondrial protein, PP2C-type mitochondrial, PP2CM, Protein phosphatase 2C isoform kappa

Molecular Weight: 41 kDa

Gene ID: 152926, 9606

UniProt: Q8N3J5