Aurora A antibody

Catalog No. ABIN498104

The Rabbit Polyclonal anti-Aurora A antibody is suitable to detect Aurora A in samples from Human, Mouse and Rat. It has been validated for WB and IHC (p).

Quick Overview for Aurora A antibody (ABIN498104)

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aurora A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Aurora A Antibody

Specificity: This antibody antibody detects endogenous levels of of AurA protein.

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: Western Blots: 1/500approx. 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2 containing 0.05 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Aurora A

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Alternative Name: Aurora Kinase A

Background: Aurora 2/AIK, a cell cycle-regulated serine/threonine protein kinase, is overexpressed in many tumor cell lines. Phosphorylation of threonine 288, which is within the activation loop of the kinase, results in a significant increase in its enzymatic activity. Aurora 2/AIK is regulated by proteasome-dependent degradation, and phosphorylation on Thr288 may target it for degradation during mitosis. Thus, phosphorylation of Aurora 2/AIK at Thr288 is important for both kinase activity and stability.Synonyms: AIK1, ARK1, AURA, AURKA, AURORA2, Aurora/IPL1-related kinase 1, BTAK, Breast tumor-amplified kinase, STK15, STK6, Serine/threonine kinase 15, Serine/threonine-protein kinase 6

Molecular Weight: approx. 48 kDa

Gene ID: 6790

NCBI Accession: NP_003591

UniProt: O14965

Pathways: Cell Division Cycle, Asymmetric Protein Localization