p300 antibody

Catalog No. ABIN498122

The Rabbit Polyclonal anti-p300 antibody is suitable to detect p300 in samples from Human. It has been validated for WB and IHC (p).

Quick Overview for p300 antibody (ABIN498122)

Target: p300 (EP300) (E1A Binding Protein P300 (EP300))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This p300 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-p300 Antibody

Specificity: This antibody detects endogenous levels of EP300 / P300 protein.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen

Application Details

Application Notes: Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for p300

Target: p300 (EP300) (E1A Binding Protein P300 (EP300))

Alternative Name: EP300 / P300

Background: KAT3B / p300 is a transcriptional adapter protein (300 kDa) which is characterized by three cysteine and histidine rich regions and its C-terminus specifically binds the adenovirus E1A protein. KAT3B and associated proteins are components of TATA-binding protein (TBP) complexes. Protein kinase A mediated CREB phosphorylation results in the binding of CREB to a 265 kDa nuclear protein designated KAT3A / CBP (for CREB-binding protein). KAT3B and KAT3A are homologous to each other.Synonyms: E1A binding protein p300, E1A-associated protein p300, Histone acetyltransferase p300, KAT3B, p300 HAT

Molecular Weight: approx. 264 kDa

Gene ID: 2033

NCBI Accession: NP_001420

UniProt: Q09472

Pathways: p53 Signaling, Notch Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Regulation of Cell Size