TSG101 antibody

Catalog No. ABIN498353

Product Details anti-TSG101 Antibody

Target: TSG101 (Tumor Susceptibility Gene 101 (TSG101))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TSG101 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of Tsg 101 protein. (region surrounding Glu303)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for TSG101

Target: TSG101 (Tumor Susceptibility Gene 101 (TSG101))

Alternative Name: TSG101 (TSG101 Products)

Synonyms: CG9712 antibody, DmTSG101 antibody, Dmel\\CG9712 antibody, ESCRT-I antibody, Tsg101 antibody, burs antibody, dTSg101 antibody, dTsg101 antibody, dVps23 antibody, ept antibody, tsg101 antibody, vps23 antibody, TSG101 antibody, MGC76193 antibody, TSG10 antibody, VPS23 antibody, AI255943 antibody, CC2 antibody, Rw antibody, zgc:86926 antibody, tumor susceptibility 101 antibody, Tumor susceptibility gene 101 antibody, Tumor Susceptibility Gene homolog antibody, tumor susceptibility gene 101 antibody, tumor susceptibility 101a antibody, tumor susceptibility 101 L homeolog antibody, TSG101 antibody, tsg-101 antibody, tsg101 antibody, Tsg101 antibody, tsg101a antibody, tsg101.L antibody

Background: It is now well accepted that the transformation of a normal cell to one that is malignant can result from mutations in genes that encode proteins with key regulatory functions. Examples include the retinoblastoma gene product (Rb p110), p53, VHL and APC. Using a novel cloning strategy that allows the isolation of previously uncharacterized genes encoding selectable recessive phenotypes, an additional tumor suppressor gene has been identified. This gene, termed tsg 101 for tumor susceptibility gene 101, encodes a stathmin binding domain protein. When expression of this growth inhibitory gene is blocked in NIH/3T3 cells using antisense mRNA, the cells exhibit a transformed phenotype and are tumorigenic in SL6 mice.Synonyms: ESCRT-I complex subunit TSG101, Tumor susceptibility gene 101 protein

Molecular Weight: approx. 45 kDa

Gene ID: 7251

NCBI Accession: NP_006283

UniProt: Q99816